Puccinia triticina Assembly and Gene Annotation

About Puccinia triticina

Puccinia triticina causes 'wheat leaf rust', a fungal disease that infects wheat. It is the most common wheat disease and ocurrs in most wheat growing regions. The fungus is an obligate parasite producing infectious urediniospores that can infect plants hundreds of kilometers away when carried by the wind.

Picture credit: James Kolmer

Assembly

The Puccinia triticina (race 1, isolate 1-1) genome was generated and assembled by Broad Institute of MIT and released in July 2010 consisting of a 16X whole-genome shotgun assembly with a size of 162.95 Mb.

Annotation

The protein coding gene annotation of the Puccinia triticina genome was imported from GFF datafiles provided the Broad Institute of MIT. Funding for the assembly and annotation was provided by USDA Cooperative State Research, Education and Extension Service through the Microbial Genome Sequencing Program with additional funding for the BAC library end-sequencing from the Agriculture and Agri-Food Canada.

Non-coding RNA genes have been annotated using tRNAScan-SE (Lowe, T.M. and Eddy, S.R. 1997), RFAM (Griffiths-Jones et al 2005), and RNAmmer (Lagesen K.,et al 2007); additional analysis tools have also been applied.

References

1. Gene discovery in EST sequences from the wheat leaf rust fungus Puccinia triticina sexual spores, asexual spores and haustoria, compared to other rust and corn smut fungi.
   Xu J, Linning R, Fellers J, Dickinson M, Zhu W, Antonov I, Joly DL, Donaldson ME, Eilam T, Anikster Y et al. 2011. BMC Genomics. 12:161.

Other data

• Mapping of transcriptomics sequences of samples from fresh and germinated spores and infected wheat (Broad Insitute, submitted to the ENA SRA as part of study SRP000717). [View the data]
• EST sequences from Xu et al. aligned to the genome with Exonerate [View data]

More information

General information about this species can be found in Wikipedia.