Puccinia graminis Ug99 Assembly and Gene Annotation

About Puccinia graminis f. sp. tritici Ug99

Stem rust, also known as cereal rust, black rust, red rust or red dust, is caused by the fungus Puccinia graminis, which causes significant disease in cereal crops. Crop species that are affected by the disease include bread wheat, durum wheat, barley and triticale. These diseases have affected cereal farming throughout history. The annual recurrence of stem rust of wheat in North Indian plains was discovered by K. C. Mehta. Since the 1950s, wheat strains bred to be resistant to stem rust have become available. Fungicides effective against stem rust are available as well.

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(Text from Wikipedia.)

More information General information about this species can be found in Wikipedia

The sequencing, assembly and annotation of protein coding genes from the Puccinia graminis (Ug99) genome has been generated by the Broad Institute as part of their work on the Puccinia group of fungi.

Non coding RNA genes have been annotated using tRNAScan-SE (Lowe, T.M. and Eddy, S.R. 1997), RFAM (Griffiths-Jones et al 2005), and RNAmmer (Lagesen K.,et al 2007); additional analysis tools have also been applied.

A variation database has been constructed by comparing this genome with Puccinia graminis f. sp. tritici sequence read data (study PRJNA253722). Reads were mapped against the Puccinia graminis Ug99 genome using BWA (Li H.and Durbin R.Bioinformatics 2010) and variations called using SAMtools (Li H.et.al Bioinformatics 2009).

More information

General information about this species can be found in Wikipedia.