Aspergillus flavus Assembly and Gene Annotation
About the Aspergillus flavus genome
The sequencing project of A. flavus (NRRL 3357) was led by Gary Payne and Ralph Dean at North Carolina State University and funded by the Microbial Genome Sequencing Project-USDA National Research Initiative. Using the whole-genome random shotgun method, this yielded 5x coverage and 24 unassigned supercontigs.
Sequencing and first-pass automated annotation was carried out by the JCVI. This was further improved by EST data provided by USDA/ARS/SRRC (New Orleans). USDA/ARS/SRRC also provided funds for sequence closure and finishing. The final phase, manual refinement of gene annotation, was undertaken by North Carolina State University. A more detailed report can found on the A. flavus project website.
Non coding RNA genes have been annotated using tRNAScan-SE (Lowe, T.M. and Eddy, S.R. 1997), RFAM (Griffiths-Jones et al 2005), and RNAmmer (Lagesen K.,et al 2007); additional analysis tools have also been applied.
Database construction and maintenance
The Aspergillus genome sequences and annotation is maintained and curated in an Ensembl schema by the Central Aspergillus Data Repository and made available for inclusion within Ensembl Genomes. CADRE is operated at the School of Medicine at the University of Manchester. More information about the CADRE management team is provided here.
- CADRE: the Central Aspergillus Data REpository.
Mabey JE, Anderson MJ, Giles PF, Miller CJ, Attwood TK, Paton NW, Bornberg-Bauer E, Robson GD, Oliver SG, Denning DW. 2004. Nucleic Acids Res.. 32:D401-5.
General information about this species can be found in Wikipedia.
|Assembly||JCVI-afl1-v2.0, INSDC Assembly GCA_000006275.2, Jun 2015|
|Golden Path Length||36,892,344|
|Genebuild method||Generated from ENA annotation|
|Non coding genes||316|
|Small non coding genes||316|