Schizosaccharomyces japonicus vs Puccinia triticina - GCA_000151525.2 LastZ results
Schizosaccharomyces japonicus (Schizosaccharomyces japonicus yFS275, GCA_000149845.2) and Puccinia triticina - GCA_000151525.2 (Puccinia triticina, GCA000151525v2) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Fungi release 114. Schizosaccharomyces japonicus was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
Parameter | Value |
---|---|
Gap open penalty (O) | 400 |
Gap extend penalty (E) | 30 |
HSP threshold (K) | |
Threshold for gapped extension (L) | 3000 |
Threshold for alignments between gapped alignment blocks (H) | 2200 |
Masking count (M) | |
Seed and Transition value (T) | 1 |
Scoring matrix (Q) | Default: A C G T 91 -114 -31 -123 -114 100 -125 -31 -31 -125 100 -114 -123 -31 -114 91 |
Chunking parameters
Parameter | Schizosaccharomyces japonicus | Puccinia triticina - GCA_000151525.2 |
---|---|---|
Chunk size | 10,000,000 | 10,100,000 |
Overlap | 0 | 100,000 |
Group set size | 10,100,000 | 10,100,000 |
Masking options |
Statistics over 6,016 alignment blocks
Genome coverage (bp) | Coding exon coverage (bp) | |
---|---|---|
Schizosaccharomyces japonicus |
Uncovered: 10,400,324 out of 11,733,154 |
Uncovered: 5,614,924 out of 6,846,208 |
Puccinia triticina - GCA_000151525.2 |
Uncovered: 133,891,480 out of 135,343,689 |
Uncovered: 16,256,979 out of 17,522,293 |
Block size distribution
Size range | All 6,016 alignment blocks | Blocks grouped in nets | |||
---|---|---|---|---|---|
# blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
1 bp - 10 bp | 10 |
65.0 bp |
|||
10 bp - 100 bp | 1,217 |
88.7 kb |
138 |
10.7 kb |
|
100 bp - 1 kb | 4,612 |
1.4 Mb |
1,504 |
701.7 kb |
|
1 kb - 10 kb | 177 |
262.7 kb |
565 |
1.0 Mb |