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Magnaporthe poae vs Saccharomyces cerevisiae LastZ results

Magnaporthe poae (Magnaporthe poae ATCC 64411, Mag_poae_ATCC_64411_V1) and Saccharomyces cerevisiae (Saccharomyces cerevisiae S288c, R64-1-1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Fungi release 110. Magnaporthe poae was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterMagnaporthe poaeSaccharomyces cerevisiae
Chunk size10,000,00010,100,000
Overlap0100,000
Group set size10,100,00010,100,000
Masking options

Statistics over 3,920 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Magnaporthe poae

Uncovered: 38,050,814 out of 39,503,331
Covered: 1,452,517 out of 39,503,331

Uncovered: 13,289,982 out of 14,559,232
Matches: 741,978 out of 14,559,232
Mismatches: 489,922 out of 14,559,232
Insertions: 37,350 out of 14,559,232
Identity over aligned base-pairs: 58.5%

Saccharomyces cerevisiae

Uncovered: 10,541,270 out of 12,157,105
Covered: 1,615,835 out of 12,157,105

Uncovered: 7,025,504 out of 8,596,583
Matches: 897,639 out of 8,596,583
Mismatches: 640,291 out of 8,596,583
Insertions: 33,149 out of 8,596,583
Identity over aligned base-pairs: 57.1%

Block size distribution

Size range All 3,920 alignment blocks Blocks grouped in nets
# blocks Total size (incl. gaps) # nets Total size (incl. gaps)
1 bp - 10 bp
5
33.0 bp
10 bp - 100 bp
554
40.1 kb
122
10.0 kb
100 bp - 1 kb
2,976
1.1 Mb
1,201
579.4 kb
1 kb - 10 kb
384
622.4 kb
656
1.2 Mb
10 kb - 100 kb
1
34.2 kb
1
35.3 kb