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Fusarium verticillioides vs Magnaporthe poae LastZ results

Fusarium verticillioides (Fusarium verticillioides 7600, ASM14955v1) and Magnaporthe poae (Magnaporthe poae ATCC 64411, Mag_poae_ATCC_64411_V1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Fungi release 110. Fusarium verticillioides was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterFusarium verticillioidesMagnaporthe poae
Chunk size10,000,00010,100,000
Overlap0100,000
Group set size10,100,00010,100,000
Masking options

Statistics over 18,783 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Fusarium verticillioides

Uncovered: 30,790,052 out of 41,885,085
Covered: 11,095,033 out of 41,885,085

Uncovered: 8,878,039 out of 17,841,847
Matches: 5,427,302 out of 17,841,847
Mismatches: 3,321,590 out of 17,841,847
Insertions: 214,916 out of 17,841,847
Identity over aligned base-pairs: 60.5%

Magnaporthe poae

Uncovered: 28,865,268 out of 39,503,331
Covered: 10,638,063 out of 39,503,331

Uncovered: 6,645,872 out of 14,559,232
Matches: 5,035,561 out of 14,559,232
Mismatches: 2,536,065 out of 14,559,232
Insertions: 341,734 out of 14,559,232
Identity over aligned base-pairs: 63.6%

Block size distribution

Size range All 18,783 alignment blocks Blocks grouped in nets
# blocks Total size (incl. gaps) # nets Total size (incl. gaps)
1 bp - 10 bp
35
218.0 bp
10 bp - 100 bp
2,047
136.7 kb
393
27.8 kb
100 bp - 1 kb
12,966
5.3 Mb
3,222
1.5 Mb
1 kb - 10 kb
3,711
6.7 Mb
3,488
8.8 Mb
10 kb - 100 kb
24
448.0 kb
139
2.2 Mb