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Fusarium solani vs Magnaporthe poae LastZ results

Fusarium solani (Fusarium solani mpVI 77-13-4, v2.0) and Magnaporthe poae (Magnaporthe poae ATCC 64411, Mag_poae_ATCC_64411_V1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Fungi release 110. Fusarium solani was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterFusarium solaniMagnaporthe poae
Chunk size10,000,00010,100,000
Overlap0100,000
Group set size10,100,00010,100,000
Masking options

Statistics over 22,036 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Fusarium solani

Uncovered: 38,339,564 out of 51,291,997
Covered: 12,952,433 out of 51,291,997

Uncovered: 11,846,033 out of 22,633,081
Matches: 6,514,365 out of 22,633,081
Mismatches: 3,995,618 out of 22,633,081
Insertions: 277,065 out of 22,633,081
Identity over aligned base-pairs: 60.4%

Magnaporthe poae

Uncovered: 28,006,626 out of 39,503,331
Covered: 11,496,705 out of 39,503,331

Uncovered: 6,139,529 out of 14,559,232
Matches: 5,423,112 out of 14,559,232
Mismatches: 2,606,371 out of 14,559,232
Insertions: 390,220 out of 14,559,232
Identity over aligned base-pairs: 64.4%

Block size distribution

Size range All 22,036 alignment blocks Blocks grouped in nets
# blocks Total size (incl. gaps) # nets Total size (incl. gaps)
1 bp - 10 bp
52
304.0 bp
10 bp - 100 bp
2,344
156.3 kb
525
35.5 kb
100 bp - 1 kb
15,234
6.2 Mb
4,146
2.0 Mb
1 kb - 10 kb
4,378
8.0 Mb
4,121
10.2 Mb
10 kb - 100 kb
28
453.8 kb
161
2.6 Mb