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Fusarium solani vs Magnaporthe oryzae LastZ results

Fusarium solani (Fusarium solani mpVI 77-13-4, v2.0) and Magnaporthe oryzae (Magnaporthe oryzae 70-15, MG8) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Fungi release 110. Fusarium solani was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterFusarium solaniMagnaporthe oryzae
Chunk size10,000,00010,100,000
Overlap0100,000
Group set size10,100,00010,100,000
Masking options

Statistics over 23,570 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Fusarium solani

Uncovered: 38,047,148 out of 51,291,997
Covered: 13,244,849 out of 51,291,997

Uncovered: 11,286,118 out of 22,633,081
Matches: 7,311,016 out of 22,633,081
Mismatches: 3,711,543 out of 22,633,081
Insertions: 324,404 out of 22,633,081
Identity over aligned base-pairs: 64.4%

Magnaporthe oryzae

Uncovered: 28,436,702 out of 41,027,733
Covered: 12,591,031 out of 41,027,733

Uncovered: 6,453,518 out of 16,744,523
Matches: 6,552,700 out of 16,744,523
Mismatches: 3,268,286 out of 16,744,523
Insertions: 470,019 out of 16,744,523
Identity over aligned base-pairs: 63.7%

Block size distribution

Size range All 23,570 alignment blocks Blocks grouped in nets
# blocks Total size (incl. gaps) # nets Total size (incl. gaps)
1 bp - 10 bp
46
256.0 bp
10 bp - 100 bp
2,387
159.0 kb
484
33.3 kb
100 bp - 1 kb
15,643
6.7 Mb
4,340
2.1 Mb
1 kb - 10 kb
5,491
9.2 Mb
5,039
11.8 Mb
10 kb - 100 kb
3
45.0 kb
131
2.1 Mb