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Ashbya gossypii vs Magnaporthe poae LastZ results

Ashbya gossypii (Ashbya gossypii ATCC 10895, ASM9102v1) and Magnaporthe poae (Magnaporthe poae ATCC 64411, Mag_poae_ATCC_64411_V1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Fungi release 110. Ashbya gossypii was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterAshbya gossypiiMagnaporthe poae
Chunk size10,000,00010,100,000
Overlap0100,000
Group set size10,100,00010,100,000
Masking options

Statistics over 2,372 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Ashbya gossypii

Uncovered: 8,267,870 out of 9,119,312
Covered: 851,442 out of 9,119,312

Uncovered: 6,184,782 out of 7,019,450
Matches: 496,836 out of 7,019,450
Mismatches: 313,307 out of 7,019,450
Insertions: 24,525 out of 7,019,450
Identity over aligned base-pairs: 59.5%

Magnaporthe poae

Uncovered: 38,491,340 out of 39,503,331
Covered: 1,011,991 out of 39,503,331

Uncovered: 13,665,360 out of 14,559,232
Matches: 544,178 out of 14,559,232
Mismatches: 312,112 out of 14,559,232
Insertions: 37,582 out of 14,559,232
Identity over aligned base-pairs: 60.9%

Block size distribution

Size range All 2,372 alignment blocks Blocks grouped in nets
# blocks Total size (incl. gaps) # nets Total size (incl. gaps)
1 bp - 10 bp
7
46.0 bp
10 bp - 100 bp
404
28.7 kb
147
10.7 kb
100 bp - 1 kb
1,690
635.2 kb
789
396.4 kb
1 kb - 10 kb
271
425.4 kb
397
682.1 kb