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Ashbya gossypii vs Magnaporthe oryzae LastZ results

Ashbya gossypii (Ashbya gossypii ATCC 10895, ASM9102v1) and Magnaporthe oryzae (Magnaporthe oryzae 70-15, MG8) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Fungi release 110. Ashbya gossypii was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterAshbya gossypiiMagnaporthe oryzae
Chunk size10,000,00010,100,000
Overlap0100,000
Group set size10,100,00010,100,000
Masking options

Statistics over 2,359 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Ashbya gossypii

Uncovered: 8,248,857 out of 9,119,312
Covered: 870,455 out of 9,119,312

Uncovered: 6,155,194 out of 7,019,450
Matches: 529,815 out of 7,019,450
Mismatches: 307,067 out of 7,019,450
Insertions: 27,374 out of 7,019,450
Identity over aligned base-pairs: 61.3%

Magnaporthe oryzae

Uncovered: 39,965,305 out of 41,027,733
Covered: 1,062,428 out of 41,027,733

Uncovered: 15,714,166 out of 16,744,523
Matches: 621,815 out of 16,744,523
Mismatches: 363,242 out of 16,744,523
Insertions: 45,300 out of 16,744,523
Identity over aligned base-pairs: 60.3%

Block size distribution

Size range All 2,359 alignment blocks Blocks grouped in nets
# blocks Total size (incl. gaps) # nets Total size (incl. gaps)
1 bp - 10 bp
2
14.0 bp
10 bp - 100 bp
339
23.4 kb
75
5.4 kb
100 bp - 1 kb
1,762
713.2 kb
808
434.6 kb
1 kb - 10 kb
256
395.3 kb
404
691.9 kb